2.4. Inoculum size on acetic fermentation
Study on the inoculum size for acetic fermentation was performed to elucidate its
effect on the acetic acid production in coconut water medium. The inoculum was
prepared by introducing 10% of A. aceti stock culture in fresh alcoholic coconut
water medium and fermented for 3 days. The concentrations of inoculum were
varied in the range of 5%, 10%, 15% and 25% (v/v) and were introduced in new
alcoholic coconut water medium. All the samples were incubated at 30oC and
shaken at 150 rpm for 4 weeks