Different pretreatment methods have been proposed so far to deconstruct the plant cell wall structure, remove and/or mod-ify both lignin and hemicellulose components, and enhancethe accessibility of glucans to enzymatic hydrolysis, this with-out compromising the fermentation yield by releasing inhibitorycompounds in the reaction system [12–14]. In this regard, the pre-treatment step has a strong influence in the overall process costsby determining the extent of sugar recovery, fermentation toxic-ity, enzymatic hydrolysis rates, enzyme loadings, and other processvariables