Prebiotic effect of inulin determinationThe response as prebiotic was evaluated using a culture mediawith the same composition of MRS broth but replacing the carbo-hydrate source for inulin extracted. This bacterial growth was alsoevaluated in the prepared MRS broth without carbohydrate source,which was used as control. The carbohydrate concentration in allthe assays was 5 g/L. The activated probiotics was inoculated with1% (v/v) and incubated at 37◦C for 32 h, when the growth of mixedstrains was in the stationary phase. The OD and pH were measuredto evaluate the growth of probiotics. All assays were performed induplicate.