2. Materials and methods
2.1. Study sites
This study was conducted at the North-West University
Experimental Farm (Molelwane), Mafikeng (25.81S and
25.51E), South Africa. M. oleifera leaves were obtained from
Patience Wellness Centre in Limpopo Province (24.3051S
29.5651E). The ambient temperature in this area ranges from 27 to 37 1C during summer and between 11 and 17 1C during winter. The annual rainfall ranges between 500 mm and 800 mm. The leaves were air-dried at a room tem- perature and then milled to pass through a 2 mm sieve.