In this study, to reduce both the raw material costs and the processing
costs for biodiesel production the feasibility to convert the
above industrial wastes into lipid and the use of direct transesterification
of the obtained lipid into biodiesel were studied. Several
oleaginous yeasts were cultivated with the industrial wastes and
screened for their abilities to grow and produce lipid from the
wastes. The combinations of the wastes were also tested to reduce
any additional costs of adding costly other nutrients. In addition, a
two-phase strategy for cell growth and lipid accumulation was attempted to increase the lipid production by the selected yeast.
Finally, the direct transesterification of the yeast lipid into biodiesel
was evaluated and optimized.