In studies related to antimicrobial
Wlms, most of the workers used commercial
lysozyme obtained by the classical repeated salt crystallization
method which may require a week until the
enzyme is obtained with suYcient purityThe commonly used commercial lysozymes
are quite pure. They are reported to contain only
1–6% (w/w) protein impuritiesand they have a very high enzyme activity
(between 20,000 and 100,000U/mg). However, for the
application of lysozyme in food industry, the use of
cheaper partially puriWed lysozyme preparations
obtained by some faster methods may be economically
more feasible. For this reason, some fast partial puriWcation
procedures have recently been developed based
on heat induced precipitation of non-lysozyme protein
impurities in presence of reductants