The aim of this reserch were investigate the substitution of sawdust with Vetiveria zizaniodes (L.)Nash, Sri Langka cultivar on
the media in mycelia growth of Ganoderma lucidum in plastic bag. The suitable three media from eleven formulas with
different weight ratio of sawdust and V. zizaniodes leaves at 100:0, 0:100 and 20:80 are selected in this research and gave the
biological efficiency with 42.72, 00.00 and 40.71% respectively. This results show the opportunity for substitution of sawdust with V.
zizaniodes leaves which can reduce the production cost of G. lucidum. Nutrition analysis of fruiting body of mushroom are cultivated
on different type of the substrates, particularly sawdust 100:0 in plastic bag, found more protein than cultivated in sawdust 20:80 with
10.29 and 8.45 mg/100 g dry weight in G. lucidum respectively. In amount of essential amino acid and non-essential amino acid of G.
lucidum cultivated sawdust 100:0 gave more amino acid than 20:80.