The Kefir grains were separated by filtration, and the fermentate was used as a natural starter culture in kefir production. Milk samples were inoculated with the natural starter culture and incubated at 25 C for approximately 20 h until pH 4.6 was reached. Kefir samples were stored at 4 C for a day. The information regarding sample design is shown in Table 1. Both Kefir samples and milk samples were analyzed.