Carnivorous plants capture and digest prey to obtain additional nutrients. Therefore, different trapping
mechanisms were developed in different species. Plants of the genus Nepenthes possess pitfall-traps filled
with a digestive fluid, which is secreted by the plants themselves. This pitcher fluid is composed of various
enzymes to digest the captured prey. Besides hydrolytic enzymes, defense-related proteins have
been identified in the fluid. The present study describes the identification and heterologous expression
of a pathogenesis-related protein, NmPR-1, from pitchers of Nepenthes mirabilis with features that are
unusual for PR-1 proteins. In particular, it was proven to be highly glycosylated and, furthermore, it
exhibited antibacterial instead of antifungal activities. These properties are probably due to the specific
environment of the pitcher fluid.