larvae (60.4+5.7/min). The injected larvae
deposited wet and sticky faeces and some of
them were attached together. The lethal
effect of VAA injection appeared at the late
stage of the larva. Eighty percent of 15
larvae became larval-pupal intermediate (pupal
cuticle was formed under the larval cuticle,
but was not shed), 13% of the larvae shed
partially larval cuticle, and the remaining one
larva pupated with the marginal part of wing
pad separated from body cuticle. All of the
treated individuals with abnormality died
afterward.
2. Inhibition of the Trehalase Activity
Trehalase activity of midgut of the control
larvae was determined to be 2.8, umol/midgut/
20 min as produced glucose. The activity of
the midgut of VAA treated larvae reduced to
only 10% of the control (average of 2 replications)
in a day.
3. Components of Laval Haemolymph
On 1H NMR spectrum of the haemolymph
of the 3rd day last instar larvae (Fig. 2(a)), 10
chemicals were assigned: leucine (L), isoleucine
(iL), valine (V), lactate (La), alanine