Edible films have been in use for more than 100 years. It started with the application of wax coatings to fruit and vegetables and now includes active edible films with selectivity toward mass transfer, light barrier, and antimicrobial activity. The market for edible films and coatings has experienced remarkable growth over the last 5 years. Accompanying this growth is a vast amount of knowledge on edible films and coatings acquired through research and product development work as well as advances in material science and processing technology. Recently, increasing attention has been given to developing and testing films with antimicrobial properties to improve food safety and shelf life. Active biomolecules such as chitosan and its derivatives have a significant role in the food packaging area, as it is a biodegradable, biocompatible, nontoxic, and antimicrobial agent with excellent film properties. All this made it an active member in the food packaging industry. However, chitosan edible films still suffers from high water vapor permeation, which lowers its protective action. Therefore, the addition of oregano essential oil increases its hydrophobicity and improves its water vapor permeation. Oregano essential oil has high levels of carvacrol, thymol, γ-terpinene, and p-cymene and shows a high level of antimicrobial activity against different strains of Gram-positive and Gram-negative bacteria. This chapter traces chitosan oregano essential oil blends in recent research for the production of antimicrobial packaging