Using the antisense gene-specific primer 5VCCACTTCATAGACTGTGCGAAGTGC-
3V derived from
the 5V-end and the sense primer 5V-GGCAATGCCAAGAAGCTCATCAAG-
3V deduced from the 3V-end of the
coding sequence within the 1.85-kb genomic fragment,
two fragments of 0.9 and 1.4 kb were amplified which
represented the 5V- and the 3V-ends of the hydA cDNA,
respectively.