Acid and sugar measurement
Acid and sugar concentrations were determined by spectrophotometry (Xenius, Safas, Monaco) by determining concentrations of NADPH and NADH respectively, at 340 nm using enzymatic kits. Enzymatic kits for citric acid (reference: 10.139.076), malic acid (reference: 10.139.068), glucose (reference: 10.716.251), fructose (reference: 10.139.106) and sucrose (reference: 10.716.251) were obtained from R-Biopharm
Acid and sugar measurementAcid and sugar concentrations were determined by spectrophotometry (Xenius, Safas, Monaco) by determining concentrations of NADPH and NADH respectively, at 340 nm using enzymatic kits. Enzymatic kits for citric acid (reference: 10.139.076), malic acid (reference: 10.139.068), glucose (reference: 10.716.251), fructose (reference: 10.139.106) and sucrose (reference: 10.716.251) were obtained from R-Biopharm
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