Isolation of bacteria from soil sample:
1 gm. soil sample was aseptically inoculated in 100 ml sterile pectin broth flask and this flask was incubated in rotary shaker for
7 days at 370C for enrichment.For isolation a loop full of enriched sample was streaked on nutrient agar (Nutrient broth + 2% agar agar).
Then plates were incubated at 370C for 24 hours. After incubation plates were checked for growth of bacterial colony.