Chemical and thermal nociceptive stimulus were given 10min after the morphine and/or coniine application. Initially drug applications were performed solitarily and effects of morphine and coniine were assessed apart from each other.
Then, morphine and coniine were applied in coincidence in order to assess their possible interactions in antinociception.
In order to evaluate the possible role of nicotinic receptors on antinociception, saline and standard drug group received mecamylamine alone.