The benefits of high pressure processing (HPP) for microbial inactivation in food production include reduced
thermal treatment and minimized effects on sensory and nutritional profiles. These benefits have resulted in
increasing commercial production of high pressure pasteurized foods. In this review, the current state of the
art in terms of vegetative cell and bacterial spore inactivation by HPP in complex food matrices is assessed
with an emphasis on mechanisms of inactivation and treatment of products that have low or non-uniform
water activity (aw) profiles. Low aw can be the result of a high concentration in solutes, the presence of oils/
fats, or the physical removal of water through dehydration. Microbial inactivation in low aw environments
remains a particular challenge for HPP and studies on microbial inactivation observed in the different types of
low aw food matrices are reviewed in detail.
Industrial relevance: HPP-treated food products with low aw have been on the market since the nineties, but the
mechanisms of microbial inactivation at low aw are still not well understood, which hinders the development of
new applications in low or inhomogeneous aw food. This review summarizes the state of the art in terms of HPP
microbial inactivation mechanisms in model systems and various low aw food environments. Thereby, it
identifies existing and potential new applications as well as the current gaps and future research needs.