Sephadex LH-20 is a liquid chromatography
media designed for molecular sizing of natural
products such as steroids, terpenoids, lipids and
low molecular weight peptides (up to 35 amino
acid residues).
Depending on the chosen solvents, this media can
also separate sample components by partition
between the stationary and mobile phases.
Sephadex LH-20 is useful at both analytical and
industrial scale for the preparation of closely
related molecular species. Due to the unique
physico-chemical properties of this media, it can
be used either during initial purification prior to
polishing by high performance ion exchange or
reversed phase chromatography or as the final
polishing step e.g. during the preparation of
diastereomers.