Wine samples were obtained from one vineyard and one producer in the Zernoseky wine region (North Bohemia, Czech Republic) during the wine-making process. Two white varieties (Muller-Thurgau ["MT"] and Moravian Muscat ["MM"]),and two blue varieties, (Blauer Portugieser ["BP"] and St. Laurent ["ST"]) were assessed. Samples were collected every week from the beginning of November 2005 until the beginning of April 2006 (21 week). In the middle of April the wines were bottled.
Spectrophotometric measurement was performed on a in Helios instrument (Spectronic Unicam,GB). The results in every assay were obtained from five parallel determinantions. The antioxidant activities were expressed in equivalents of ascorbic acid mg/ml (EQA), which could provide the same inactivation as the studied sample volume.