Table 1 shows the antimicrobial activity of fresh-cut mango untreated and treated with the ethanolic extract of seeds, stored at 5°C during 15 days. A significant effect (p ≤ 0.05) of the treatment on the growth of the mesophilic aerobic microorganisms and total fungi and yeast was found. On days 0 and 5 microbial counts were under the limit of enumeration, however, on days 10 and 15 the control had a higher population of these microorganisms, being statistical differences amongst them. At the end of the storage, the treatment inhibited the growth of mesophilic aerobic microorganisms, total fungi and yeast causing an 80% and 97% of microbial reduction compared to control fruit.