After both the best amount of C.tropicalis and gelatine were determined as 4.42 mg cm for the investigation of the effect of the cross-linking agent,glutaraldehyde on the biosensor response the amount of C.tropicalis and gelatine were kept constant as 4.42 mg cmand each of the newly prepared microbial biosensors weretreated with 0.05, 0.1, and 0.2% glutaraldehyde