Applicable to all types of fixed tissues for use in clinical and/or research endeavors
Archival specimens can be used (up to at least 7 years old)
Architecture of specimen remains intact
Amenable to automated analysis
Manual review of the captured images is straightforward
Requires approximately equal time to conventional fluorescence in situ hybridization interpretation
Reliable and unbiased results
Fast (approximate turn-around-time of 48–72 h)
Variety of fixatives can be used, although with variable success
Formalin and acetic acid zinc formalin (>95% success)
B5 and decalcified bone marrow core biopsy (less than or equal to50% success, although small sample size in our study)