The minimum microbicidal concentration (MMC), which
includes minimum bactericidal (MBC) and minimum fungicidal
concentrations (MFC), of the essential oil was determined according
to the MIC values. The samples showing no increases in
turbidity were streaked on nutrient agar medium and incubated for
24 h at 37 C for bacteria, 28 h at 28 C for yeasts and 24 h at 28 C
for moulds. The lowest concentration in the medium which had
fewer than five colonies was taken as the minimum microbicidal
concentration (MMC) (Li et al., 2012; Tenore et al., 2011).