Results and discussion
Hydrodistillation in a Clevenger-type apparatus insures isola-
tion and concentration of free volatile compounds and volatile
aglycones obtained by degradation of glucosinolates during isola-
tion.
O
-Glycosidically-bound volatiles were water extracted and
purified by flash chromatography.
Volatile compounds, isolated from the leaves and roots of
Raph-
anus sativus
var. black, white and red, as well as
O
-aglycones ob-
tained by hydrolysis with
b
-glucosidase from
O
-glycosides, were
subjected to a detailed GC and GC–MS analyses. Structure and mass
spectraldataof isothiocyanates andnitrilesformed byglucosinolate
degradation identified in the leaves and/or roots of different varie-
ties of
Raphanus sativus
L. are given in
Table 2
. Roots were found
nottocontainvolatilesboundas
O
-glycosides.Theyieldsofobtained
volatiles were from fresh leaves 510–1880 mg/kg with identifica-
tion from 91.7% to 97.8%; from root 359–2440 mg/kg with identifi-
cation from 89.9% to 96.8%; from leaves as
O
-glycosidically bound
volatiles 69–112 mg/kg with identification from 80.5% to 84.5%.