Each byproduct fraction was analysed separately. After processing, the matrices were packaged, stored for 2 days at 5 C, crushed mechanically to a particle volume of approximately 0.5 cm
, frozen and lyophilised for 48 h. After lyophilisation, the matrices were again crushed and stored at approximately 7 C until the time of analysis (until 6 months after the sampling). Lyophilisation was carried out to remove water from the system, increase the enzyme concentration and allow for homogenisation. The particle
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