2. Materials and methods
2.1. Nematode inoculum preparation
Infected sample was collected from a tomato field in Shiraz (Fars province, Iran) and single egg mass was used to establish a population on Rutgers tomato variety for the experiment. Eggs were extracted from infected tomato roots using 1% NaOCL. Extracted eggs were gently washed with tap water to remove NaOCL (Hussey and Barker, 1973). The species of nematode was identified as M. javanica according to morphological and morphometrical characters (Eisenback, 1985).