In this study, we obtained extracts from Brazilian cherry (or Pitanga) seeds using supercritical CO2(SC-CO2) andanalyzed the volatiles profile. Seeds were placed in contact with the SC-CO2under different conditions of pressure(P) and temperature (T). The yield, the refractive index, the presence of terpenes by thin layer chromatography (TLC)and the volatiles profile by gas chromatography with mass spectrometry (GC–MS) were determined for all the extracts.With respect to the extract yield (0.16–0.48 g/100 g), surface response analysis revealed that the greatest yield wasobtained at high P and T did not affect the process, which indicated that low temperatures can be used to protectthe thermosensitive substances. The presence of terpenes with antioxidant activity was detected via TLC analysis,and the GC–MS results indicated -elemene and germacrone as the major components and the concentration of thesesquiterpenes in the extract was influenced by the interaction between P and T.