Fatty acid analysis was performed according to Ka¨mpfer &
Kroppenstedt (1996). The fatty acid composition of strain
CC-SPL15-2T was similar to those of members of the genus
Salinicoccus and most similar to the fatty acid composition
of S. hispanicus CCUG 43288T, but some quantitative
differences were observed (Table 1). The characteristic fatty
acids anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were
detected in strain CC-SPL15-2T.
The results of the comparative physiological characterization
using almost-identical test conditions (Ka¨mpfer et al., 1991)
for strain CC-SPL15-2T and members of the genus
Salinicoccus are given in Table 2 and the species description.
DNA–DNA hybridization between strain CC-SPL15-2T and
S. hispanicus CCUG 43288T was done according to the
method of Ziemke et al. (1998) and revealed 42 and 32%
(reciprocal analysis) DNA–DNA relatedness.
Our data demonstrate that strain CC-SPL15-2T exhibits
traits that are typical of the genus Salinicoccus: for example,
the diagnostic diamino acid lysine, a quinone system
comprising predominantly menaquinone MK-6 and a
polar lipid profile consisting of diphosphatidylglycerol,
phosphatidylglycerol and an unidentified glycolipid. The
isolate could be distinguished from its closest relative S.
hispanicus CCUG 43288T and other members of the genus
Salinicoccus by physiological traits, DNA–DNA relatedness
and differences in 16S rRNA gene sequences. We propose
that strain CC-SPL15-2T is a representative of a novel
species, with the name Salinicoccus sesuvii sp. nov.