One hundred u1 cell suspensions of the isolated thermotolerant yeast strain taken from actively growing culture was inoculated into 7 ml of YPD liquid medium in a test tube containing a durham tube. Fermentation was recognized by the accumulation of Co2 gas trapped in the inner durham tube. All culture wasnincubated at 40 c for 3 days. The ethanol production was determined by usiing a gas chromatograph equipped with capillary PE-1 column. The analysis of ethanol was operated on flame-ionization detector and an inlet system using the splitless injection technique, injector and detector temperture were 250 and 300 c, respectively. Oven program was 37 c for 5 min and increase from 37 to 245 c at 10 c/ min. Helium gas was used as carrier gas, adjusted to 14 psi. The ethanol production was primarily identified by comparing the retention time of the gas chromatographic peak with ethanol standard.