Conclusions
We propose a new and original method to produce acetaldehyde and diacetyl for use in the bakery industry using yeast fermentation. A natural culture medium that mimics the synthetic yeast culture (YPG) was developed and used successfully to screen potential isolates and produce substantial amounts of both acetaldehyde and diacetyl. The yeasts species were identified using ID 32 C™ tests; and gas chromatography was used to confirm acetaldehyde and diacetyl production and accurately quantify the amount produced during fermentation. Although C. lipolytica was capable of synthesizing high amounts of acetaldehyde, C. globosa could biosynthesize substantial amounts of both diacetyl and acetaldehyde under neutral pH conditions. In the light of these results, we propose this system to be tested for biotechnological transfer to industry-level fermenters and to be used during the production of fermented products.