2.2. Effects of hydrocolloids on acrylamide formation in chemical
models
Chemical model reactions were carried out according to the
method of Gokmen and Senyuva (2007) with minor modifications.
In brief, the model systems contained 1 mmol of asparagine and
1 mmol of glucose, with or without a test hydrocolloid (2%, w/w).
The reaction medium was 2 ml of Milli-Q water. Two ml of silicone
oil were added to each cavity of the heating block. The heating
block was preheated to 170 C for 2 h before inserting the vials into
the cavities. The total heating time was 30 min and the temperature
was checked every 10 min. Fluctuation of temperature was
within 2 C. The vials were immediately cooled in an ice-water
bath. The content of each vial was transferred to a 50 ml Falcon
tube and the vials were then rinsed in triplicate, each with 3 ml
of deionized water. After centrifugation (8000g) for 10 min,
0.2 ml of the clear supernatant was transferred to an HPLC vial, followed
by dilution with 1 ml of deionized water. The diluted samples
were subjected to HPLC analysis. The experiment was
performed in triplicate.