Andrographis paniculata is a medical plant with short life cycle, widely found in Thailand and cheap. Numerous researchs have been studied in biological activities to treat human diseases Additionally, the tests of acute toxicity in animals have been proven that this plant is safeand can be used to prohibit microbial infection in animals. Lead to reduce production cost and antibiotic residues problem in animal products for consumers. Therefore, this seminar aine to study the potential of Andrographis paniculata to use as pharmaceuticals to prevent and treat the chicken diseases. The results showed that the plant in 75% Methanol extract had the high efficiency to inhibit pathogenic bacteria (Staphylococcus aureus ATCC 25923, Staphylococcus aureus BHU 011and Enterococcus faecalis ) in digestive tract of chickens when compared to the water extract. However,there was no effect on inhibition of those are