Technique
Immediately before use, carefully dry the agar plates, preferably with the
lids off and the agar surface downwards, in a drying cabinet, until the agar
surface is free of visible moisture (maximum 30 minutes).
Using the culture obtained from enrichment broth (Bolton Broth, Art.
No. 02-688), inoculate the mCCDA with a sterile loop. Incubate plates at
41,5ºC in a microaerobic atmosphere (approximately 5% O2, 10% CO2
and 85% N2 or H2), for 44 ± 4 hours.
Campylobacter jejuni strains produce grey, moist flat and occasionally
spreading growth which may be accompanied with a green hue and/or
a metallic sheen.
Campylobacter coli strains tend to be creamy-grey in colour, moist and
often produce a more discrete type of colony.
Campylobacter lari strains are more varied and produce both types of
colonial morphology
Occasionally contaminating organisms may grow on this medium. These
include cefoperazone-resistant Pseudo