Colorimetric [14], spectrophotometric [15] and high-performance
liquid chromatography (HPLC) methods have been used for the
analysis of L-canavanine
Chromatographic separationin the HPLC methods that have been described so far provides a much
higher selectivity, but these methods in general are time consuming
and are also hampered by the limited stability of reagents or
derivatized amino acid.
The goal of this research was to determine
whether HPLC chromatography of thee thoxymethylenemalonate
(DEEMM) derivatives of free amino acid scanbe used for determination of L-canavanine as well as other free amino acids in the seeds of
V. disperma.
DEEMM is auniversal reagent for amino groups and has
been used in amino sugar [19] and amino acid [20] chemistry,as well
as for amino acid analysis