The tissue samples of liver, kidney, spleen, intestine, gill, and muscle of rockfish were performed with freeze-dried to measure dry weight of the samples. The freeze-drying samples were digested by a wet digestion method (Arain et al., 2008 and Korai et al., 2008). The dried samples were digested in 65%(v/v) HNO3, and re-dried at 120 °C on hot plate. The procedure was repeated until total digestion. The entirely digested samples were diluted in 2%(v/v) HNO3. The samples were filtered through a 0.2 µm membrane filter (Advantec MFS, Inc.) under pressure for analysis. For determination of total lead concentrations, the digested and extracted solutions were analyzed by ICP-MS. The ICP-MS measurements were performed using an ELAN 6600DRC ICP-MS instrument with argon gas (Perkin-Elmer). Total lead concentrations were determined by external calibration. ICP multi-element standard solution VI (Merck) was used for standard curve. The lead bioaccumulation in tissue samples was expressed µg/g dry wt.