The new way of producing biodiesel by using an enzyme is using the microbial cells as a biological catalyst instead of pure lipase enzyme will reduces the cost of using the lipase enzyme in the biodiesel production. (Arabmarkadeh et al., 2014). The biodiesel production using microbial cells with produced lipase enzyme as biocatalysts can reduce the cost of producing biodiesel by using lipase enzyme as a catalyst (Fukuda et al.,. 2008)