This study is focused on the development of a cosmetic matrix made of nano-emulsions enriched on long-chain polyunsaturated fatty acids (LC-PUFA) as final applica- tion products, and on the optimization of their prepara- tion. We focused on two of them: eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3).