2.1. Immunization protocol
One hundred eighty-two lactating Holstein cows (University
of Guelph Elora/Ponsonby Research herds) outside
the peripartum period were immunized on days 0
and 14 with 0.5 mg hen-egg white lysozyme (HEWL,
Sigma–Aldrich Canada Ltd., Oakville, Ontario, Canada),
0.5 mg killed Candida albicans (CaWC, crude whole cell,