RELEASE OF INFORMATION ............

RELEASE OF INFORMATION ...............................................................................................................................2
EXECUTIVE SUMMARY .........................................................................................................................................3
NOMENCLATURE ....................................................................................................................................................5
CERTIFICATION.......................................................................................................................................................6
LIST OF TABLES.......................................................................................................................................................9
LIST OF FIGURES...................................................................................................................................................11
KEY TO ABBREVIATION AND ACRONYMS....................................................................................................12
1 RATIONALE FOR DEVELOPMENT OF ARCTICTM APPLES ................................................................15
1.1 BASIS FOR REQUEST FOR DETERMINATION OF NONREGULATED STATUS UNDER 7 CFR PART 340.6....15
1.2 ARCTICTM APPLE EVENTS GD743 AND GS784..........................................................................................15
1.3 BENEFITS OF ARCTICTM APPLE EVENTS GD743 AND GS784 ...................................................................16
1.4 SUBMISSIONS TO OTHER REGULATORY AGENCIES...................................................................................16
2 THE APPLE FAMILY......................................................................................................................................17
2.1 APPLE AS A CROP........................................................................................................................................17
2.2 TAXONOMY OF APPLE ................................................................................................................................18
2.3 GENETICS OF APPLE ...................................................................................................................................19
2.4 PROPAGATION AND POLLINATION OF APPLE ............................................................................................21
2.5 WEEDINESS OF APPLE ................................................................................................................................22
2.6 MODES OF GENE ESCAPE IN APPLE ...........................................................................................................22
3 DESCRIPTION OF THE TRANSFORMATION SYSTEM.........................................................................25
4 DONOR GENES AND REGULATORY SEQUENCES................................................................................27
4.1 BIOLOGY OF POLYPHENOL OXIDASE (PPO) .............................................................................................27
4.1.1 PPO Evolutionary History...................................................................................................................27
4.1.2 Plant PPOs and Browning...................................................................................................................27
4.1.3 PPO Biochemistry and Physiological Function ..................................................................................29
4.1.4 PPO Molecular Biology.......................................................................................................................31
4.2 GEN-03 VECTOR ........................................................................................................................................34
4.3 PPO SUPPRESSION TRANSGENE.................................................................................................................36
4.4 NPTII SELECTION MARKER .......................................................................................................................36
4.5 CONCLUSIONS .............................................................................................................................................36
5 GENETIC ANALYSIS AND MOLECULAR CHARACTERIZATION.....................................................37
5.1 MOLECULAR CHARACTERIZATION............................................................................................................37
5.1.1 Polymerase Chain Reaction (PCR) .....................................................................................................37
5.1.2 Southern Blot Analysis.........................................................................................................................38
5.1.3 Reverse Primer PCR (rpPCR) .........................................................................

ผลลัพธ์ (ไทย) 1:

RELEASE OF INFORMATION ............................................................................................................................... 2EXECUTIVE SUMMARY ......................................................................................................................................... 3NOMENCLATURE .................................................................................................................................................... 5CERTIFICATION....................................................................................................................................................... 6LIST OF TABLES....................................................................................................................................................... 9LIST OF FIGURES................................................................................................................................................... 11คีย์ตัวย่อและตัวย่อ... 12เหตุผล 1 การพัฒนาของ ARCTICTM แอปเปิ้ล... 151.1 ข้อมูลพื้นฐานสำหรับการขอกำหนดสถานะ NONREGULATED ภายใต้ 7 CFR ส่วน 340.6... 151.2 กิจกรรมของ APPLE ARCTICTM GD743 และ GS784 ... 151.3 ประโยชน์ของกิจกรรมของ APPLE ARCTICTM GD743 และ GS784 ... 161.4 การส่งข้อมูลให้หน่วยงานกำกับดูแลอื่น ๆ ... 162 THE APPLE FAMILY...................................................................................................................................... 172.1 APPLE AS A CROP........................................................................................................................................ 172.2 TAXONOMY OF APPLE ................................................................................................................................ 182.3 GENETICS OF APPLE ................................................................................................................................... 192.4 การเผยแพร่และการผสมเกสรของ APPLE ... 212.5 WEEDINESS OF APPLE ................................................................................................................................ 222.6 MODES OF GENE ESCAPE IN APPLE ........................................................................................................... 22คำอธิบายเกี่ยวกับระบบการแปลง 3 ... 25ผู้บริจาค 4 ยีนและลำดับกฎระเบียบ... 274.1 ชีววิทยาของ POLYPHENOL OXIDASE (PPO) ... 274.1.1 PPO Evolutionary History................................................................................................................... 274.1.2 Plant PPOs and Browning................................................................................................................... 274.1.3 PPO ชีวเคมีและสรีรวิทยา... 294.1.4 PPO Molecular Biology....................................................................................................................... 314.2 GEN-03 VECTOR ........................................................................................................................................ 344.3 PPO SUPPRESSION TRANSGENE................................................................................................................. 364.4 NPTII SELECTION MARKER ....................................................................................................................... 364.5 CONCLUSIONS ............................................................................................................................................. 365 การวิเคราะห์ทางพันธุกรรมและสมบัติระดับโมเลกุล... 375.1 MOLECULAR CHARACTERIZATION............................................................................................................ 375.1.1 ปฏิกิริยาลูกโซ่พอลิเมอเรส (PCR) ... 375.1.2 Southern Blot Analysis......................................................................................................................... 385.1.3 กลับพื้น PCR (rpPCR) ...

ผลลัพธ์ (ไทย) 2:

การเปิดเผยข้อมูล
สรุป ของ ของ TO ABBREVIATION และ เหตุผลสำหรับการพัฒนาแอปเปิ้ล ARCTICTM ............................................ .................... 15 1.1 เกณฑ์สำหรับการเรียกหาปริมาณสถานะ NONREGULATED สังกัด 7 CFR Part 340.6 .... 15 1.2 ARCTICTM APPLE กิจกรรม GD743 และ GS784 ... .................................................. ..................................... 15 1.3 ประโยชน์ของ ARCTICTM APPLE กิจกรรม GD743 และ GS784 ... .................................................. .............. 16 1.4 การส่งให้กับหน่วยงานกำกับดูแลอื่น ๆ ............................. .................................................. .... 16 2 APPLE แอปเปิ้ล อนุกรมวิธานของแอปเปิ้ พันธุศาสตร์ของแอปเปิ้ ขยายพันธุ์และการผสมเกสรของแอปเปิ้ ............................................. ............................................... 21 2.5 ความผอมโซ แอปเปิ้ล รูปแบบของการ GENE ESCAPE แอปเปิ้ล คำอธิบายของระบบส่งถ่าย ............................................. ............................ 25 4 ยีน DONOR และลำดับการควบคุม ............... .................................................. ............... 27 4.1 ชีววิทยาของโพลีฟีนออกซิเดส (PPO) .......................... .................................................. ................. 27 4.1.1 PPO วิวัฒนาการ PPOs อาคารและ PPO ชีวเคมีและการทำงานทางสรีรวิทยา ............................................. ..................................... 29 4.1.4 PPO โมเลกุล GEN-03 vector PPO ปราบปราม NPTII คัดเลือก MARKER สรุป การวิเคราะห์ทางพันธุกรรมระดับโมเลกุลและลักษณะสมบัติ ............................................. ........ 37 5.1 อณู (Polymerase Chain Reaction PCR) ภาคใต้เปรอะเปื้อน ย้อนกลับรองพื้น PCR (rpPCR) ............................................ .............................