However, this hyper-mutability can be useful for
development of a strain to be used for industrial vinegar
fermentation, which can grow under stressful conditions.
Steiner and Sauer (14) improved the phenotype of wildtype
A. aceti to resist acetate at 50 g/L by selection within
240 generations. In this study, the ethanol resistance of A.
pasteurianus CGMCC 3089 was improved using a
continuous ethanol stress adaptation culture with focus on
the evolutionary response of ADH and ALDH in strains
during an evolutionary time-scale. Adaption of cell growth
and an improved acetic acid production resulted.