The multiplex LAMP (mLAMP) assay was performed as following
system: 25 μL of a mixture containing 2.5 μL of 10× LAMP buffer,
8mM Mg2+, 1.0 mM dNTPs mixtures (0.25 mM of each dNTP), 2%
DMSO (V/V), 20 pmol each of inner primers (FIPs and BIPs), 2.5 pmol
each of out primers (F3s and B3s), 10 pmol each of loop primers (LBs
and LFs), 8 U Bst DNA polymerase and 2 μL template DNA each of
Salmonella strains and Shigella strains.