2.2. Liposome preparation
Multilamellar liposomes were prepared by the dehydration–rehydration method (Bangham, 1972). Briefly, 40 lmol of lipids were dissolved in 500 lL ethanol in a round bottom flask, solvent was dried in a rotary evaporator at 37 C. Dry lipid film composed by SPC, SPC:SA (1:0.25, mol ratio), and SPC:CaS (1:0.25, mol ratio)was rehydrated with 2 mL distilled water to a final 50 mM lipid concentration.
2.2. Liposome preparationMultilamellar liposomes were prepared by the dehydration–rehydration method (Bangham, 1972). Briefly, 40 lmol of lipids were dissolved in 500 lL ethanol in a round bottom flask, solvent was dried in a rotary evaporator at 37 C. Dry lipid film composed by SPC, SPC:SA (1:0.25, mol ratio), and SPC:CaS (1:0.25, mol ratio)was rehydrated with 2 mL distilled water to a final 50 mM lipid concentration.
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