Fig. 5. Protein gel blot analysis of D1 protein for WT and transgenic plants under heat stress for 24 h. Thylakoid membrane proteins were separated by SDS-PAGEand then probed with D1 antibody. The same thylakoid membrane proteins wereseparated by SDS-PAGE, and stained with Coomassie Brilliant Blue R250 as a loadingcontrol for protein gel blot analysis.