Sodium Dodesyl Sulfate-Polyacrylamid gel electrophoresis (SDS-PAGE) was carried out for identifying and confirming the production of keratinase enzyme by Bacillus spp. The mineral medium containing feather was centrifuged at 2500 ×g for 20 minutes. The supernatants were loaded on SDS-PAGE. SDS-PAGE was carried out on polyacrylamide slab gel consisting of 10% separating gel and 4% stacking gel (13). The molecules with standard weights were β-Galactosidase (120 KD), Bovine Serum Albumin (85 KD), Ovalbumin (50 KD), Cardonic anhydrase (35 KD), β–lactoglobuline (25 KD) and Lysozyme (20 KD).