Fig. 1 Various stages of embryogenic callus induction in C. ternatea.
a Germination of C. ternatea seeds on MS basal medium. One seed
has already produced radicle. Bar = 1.0 cm. b A fully germinated
seed on MS basal medium just before expanded cotyledon excision.
Bar = 0.9 cm. c An excised cotyledon just after inoculation on MS
medium supplemented with 2.0 mg/l 2, 4-D. Bar = 1.0 cm. d Callus
initiation from cotyledonary explants on MS medium supplemented
with 3.0 mg/l 1 week after culture. Bar = 0.8 cm. e Proliferated
embryogenic callus on MS medium with 2.0 mg/l 2, 4-D 45 days
after culture. Bar = 0.8 cm