3.1. Efficacy of H.uvarum combined with different concentrations of SA (or SBC) on control of gray mold in grape fruit
Disease incidence and lesion diameter in all treated fruit were significantly lower than those of the control fruit (P<0.05) (Fig. 1). H. uvarum, as a stand-alone treatment, reduced the disease incidence from 93.1% (control) to 51.8%. The combination of the chemical reagents and yeast decreased the disease incidence to a lower level,38.5% for H.uvarum combined with 2mmolL−1 SAand 34.3% for H. uvarum combined with 2% SBC, respectively. At the same concentration of H. uvarum, the lesion diameter of fruit treated by 2mmolL−1 SA was significantly (P<0.05) lower than in those treated by 1mmolL−1 SA and 4mmolL−1 SA (Fig. 1) after 3 days of incubation. The lesion diameter in fruit treated by 2mmolL−1 SA together with 1×108 CFUmL−1 H. uvarum was 0.81cm,while the corresponding diameter in fruit treated by yeast alone was 0.97cm. The inhibitory effects increased by 16.5% compared to the treatment of the yeast alone. As can be seen, SA was able to increase the inhibition of H. uvarum against B. cinerea. The lesion diameter in fruit treated with 2% SBC was significantly (P<0.05) lower than in those treated with 1% SBC or 4% SBC after 3 days of incubation, when the concentration of H. uvarum was identical (Fig. 1). The lesion diameter in fruit treated with 2% SBC in combination with 1×108 CFUmL−1 H.uvarum was 0.75cm, which was 22.6% lower than that in fruit treated by H.uvarumalone. Therefore, the combined treatment of H. uvarum with 2% SBC gave a remarkably improved control of B. cinerea infections in grapes.
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