Quantitative estimation of phosphate solubilization for
selected isolate was carried out by chlorostanus-reduced
molybdo-phosphoric acid blue method [29]. HCN
production was checked by culturing the organism on
nutrient agar medium supplemented with glycine (0.44 %).
Filter paper dipped in 0.5 % picric acid was attached to the
lid of culture tube. After incubation for 48 h at 28 ± 1 C,
colour change was observed for HCN production [4].
Siderophore production was detected qualitatively on
Chrome-Azurol S agar (CAS) medium [21].