a b s t r a c t
Orchids are one of the promising medicinal plant families of which Dendrobium crepidatum. Lindl &
Paxton figures out prominently because of its multi directional medicinal attributes. In the present report,
in vitro regeneration protocol has been developed from the nodal segments of D. crepidatum, to cater
sustainable commercial exploitation and conservation needs. Thidiazuron (TDZ) at 3 mg/l singly resulted
in the response frequency of 55% which could be increased to 97% by incorporating NAA at 0.5 mg/l
and TDZ at 2 mg/l in combination in the medium. Shoot induction rate was further enhanced with the
use of polyamines and at 0.8 mM putrescine along with 2 mg/l TDZ and 0.5 mg/l NAA in the medium
11.8 shoots/explant could be obtained. Highest rooting frequency of the shoots was achieved in medium
containing 2 mg/l IBA. Genetic stability of the acclimatized plants was assessed using Start Codon Targeted
(SCoT) polymorphism and inters simple sequence repeats (ISSR).