In addition, leaf anthocyanins are always biosynthesized de novo in autumn or winter in conjunction with low-temperature and short-day conditions in temperate zones.
To investigate the role of day length in anthocyanin biosynthesis under low temperatures, we selected Begonia semperflorens ‘Super Olympia’, a perennial evergreen herb, as the plant for our study
because its leaves become red in autumn. A normal temperature was used as a control, and we analyzed the different effects on anthocyanin accumulation between long-day and short-day light regimes under low temperature, as well as the
mechanisms underlying these differences.