Paw edema induced to produce acute inflammation Carrageenan-, 5-HT-, and bradykinin-induced edema was adopted as introduced by Winter, Risley, and Nuss 1962 with slight modification by Ghosh and Singh.[16] Carrageenan 1% in 0.9 % w/v sodium chloride was taken and 0.1 ml of this solution was injected in subplanter region of hind paw of all the rats of set A, one hour after the administration of the drugs. The paw volume was measured after 1 hour and again after 3 hours using a plethysmometer. Increase in volume of paw edema was recorded by subtracting the initial paw volume from final paw volume measured after 1 hour and 3 hours of carrageenan injection. Percentage of inflammation rate was calculated as