2.4. Temperature optimization of lipase catalyzed reaction
Methanol (162 lL) was added to a mixture of waste lipid (1 g) and lipase (0.1 g, 10 wt.%). The reactions were carried at different temperatures (30 _C, 40 _C, 50 _C and 60 _C). After 6 h of reaction the sample and enzyme was separated by filtration. The resulting samples were then dried and analyzed by 1H NMR.